Top 500 NEET Biology MCQs (2025)

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Top 500 NEET Biology MCQs (2025)

Q1. Which organelle is called the "powerhouse of the cell"?

(a) Golgi apparatus

(b) Mitochondria

(c) Nucleus

(d) Lysosome

Answer: (b) Mitochondria


Q2. The fluid mosaic model is related to:

(a) Cell wall

(b) Plasma membrane

(c) Nucleus

(d) Ribosome

Answer: (b) Plasma membrane


Q3. Which of the following is not a part of the endomembrane system?

(a) Golgi body

(b) Mitochondria

(c) ER

(d) Lysosome

Answer: (b) Mitochondria


2. Genetics & Evolution

Q4. Who is known as the "Father of Genetics"?

(a) Charles Darwin

(b) Gregor Mendel

(c) T.H. Morgan

(d) Watson & Crick

Answer: (b) Gregor Mendel


Q5. A cross between two heterozygous tall plants (Tt × Tt) will produce what ratio of tall to dwarf plants?

(a) 3:1

(b) 1:2:1

(c) 1:1

(d) 9:3:3:1

Answer: (a) 3:1


Q6. Which enzyme is used to join DNA fragments?

(a) DNA polymerase

(b) RNA polymerase

(c) Ligase

(d) Helicase

Answer: (c) Ligase


Q7. Which hormone regulates blood glucose levels?

(a) Insulin

(b) Thyroxine

(c) Adrenaline

(d) Estrogen

Answer: (a) Insulin


Q8. The functional unit of the kidney is:

(a) Neuron

(b) Nephron

(c) Alveoli

(d) Villi

Answer: (b) Nephron


Q9. Oxygen is transported in blood mainly by:

(a) Plasma

(b) Platelets

(c) Hemoglobin

(d) Lymph

Answer: (c) Hemoglobin


Q10. The process of water movement through a plant is called:

(a) Transpiration

(b) Photosynthesis

(c) Respiration

(d) Translocation

Answer: (a) Transpiration


Q11. Which pigment is responsible for photosynthesis?

(a) Melanin

(b) Chlorophyll

(c) Hemoglobin

(d) Xanthophyll

Answer: (b) Chlorophyll


Q12. The opening and closing of stomata are regulated by:

(a) Guard cells

(b) Xylem

(c) Phloem

(d) Root pressure

Answer: (a) Guard cells


Q13. The ozone layer protects us from:

(a) UV rays

(b) Infrared rays

(c) X-rays

(d) Gamma rays

Answer: (a) UV rays


Q14. Which gas is a major greenhouse gas?

(a) Oxygen

(b) Nitrogen

(c) Carbon dioxide

(d) Hydrogen

Answer: (c) Carbon dioxide


Q15. The first trophic level in a food chain is always:

(a) Herbivores

(b) Carnivores

(c) Producers

(d) Decomposers

Answer: (c) Producers


Q16. In humans, fertilization occurs in the:

(a) Uterus

(b) Ovary

(c) Fallopian tube

(d) Vagina

Answer: (c) Fallopian tube


Q17. Which cells produce testosterone?

(a) Sertoli cells

(b) Leydig cells

(c) Granulosa cells

(d) Oocytes

Answer: (b) Leydig cells


Q18. The placenta is derived from:

(a) Maternal tissue only

(b) Fetal tissue only

(c) Both maternal and fetal tissue

(d) None of the above

Answer: (c) Both maternal and fetal tissue


Q19. PCR stands for:

(a) Polymerase Chain Reaction

(b) Protein Coding Region

(c) Photosynthetic Carbon Reduction

(d) Polymerase Coding Region

Answer: (a) Polymerase Chain Reaction


Q20. The vector used in human insulin production is:

(a) Agrobacterium

(b) E. coli

(c) Bacillus thuringiensis

(d) Rhizobium

Answer: (b) E. coli


Q21. Which of the following is a viral disease?

(a) Tuberculosis

(b) Malaria

(c) AIDS

(d) Typhoid

Answer: (c) AIDS


Q22. The vaccine for smallpox was developed by:

(a) Louis Pasteur

(b) Robert Koch

(c) Edward Jenner

(d) Alexander Fleming

Answer: (c) Edward Jenner


9. Diversity in Living Organisms

Q23. Binomial nomenclature was introduced by:

(a) Darwin

(b) Linnaeus

(c) Lamarck

(d) Mendel

Answer: (b) Linnaeus


Q24. Which is the largest phylum in the animal kingdom?

(a) Mollusca

(b) Arthropoda

(c) Chordata

(d) Annelida

Answer: (b) Arthropoda


Q25. The tissue responsible for growth in plants is:

(a) Parenchyma

(b) Collenchyma

(c) Sclerenchyma

(d) Meristematic

Answer: (d) Meristematic


Q26. Which type of epithelium is present in the lining of blood vessels?

(a) Squamous

(b) Cuboidal

(c) Columnar

(d) Ciliated

Answer: (a) Squamous


Q27. Which biomolecule is the main energy source for cells?

(a) Proteins

(b) Lipids

(c) Carbohydrates

(d) Nucleic acids

Answer: (c) Carbohydrates


Q28. Enzymes are made up of:

(a) Carbohydrates

(b) Proteins

(c) Fats

(d) Vitamins

Answer: (b) Proteins


Q29. The enzyme that digests starch is:

(a) Pepsin

(b) Amylase

(c) Lipase

(d) Trypsin

Answer: (b) Amylase


Q30. The hormone responsible for ovulation is:

(a) Progesterone

(b) Estrogen

(c) LH (Luteinizing Hormone)

(d) FSH

Answer: (c) LH


Q31. The fertilized egg is called:

(a) Zygote

(b) Blastocyst

(c) Morula

(d) Gastrula

Answer: (a) Zygote


Q32. The male gamete in humans is produced in the:

(a) Epididymis

(b) Vas deferens

(c) Seminiferous tubules

(d) Prostate gland

Answer: (c) Seminiferous tubules


Q33. Pollen grains are produced in the:

(a) Ovary

(b) Anther

(c) Stigma

(d) Style

Answer: (b) Anther


Q34. Parthenocarpy results in the formation of:

(a) Seedless fruits

(b) Seeds without fertilization

(c) Polyploidy

(d) GMOs

Answer: (a) Seedless fruits


Q35. The fusion of male and female gametes in plants is called:

(a) Pollination

(b) Fertilization

(c) Germination

(d) Double fertilization

Answer: (d) Double fertilization


14. Evolution

Q36. Darwin’s theory of natural selection is based on:

(a) Inheritance of acquired characters

(b) Survival of the fittest

(c) Use and disuse of organs

(d) Mutation theory

Answer: (b) Survival of the fittest


Q37. Which evidence supports evolution?

(a) Fossils

(b) Analogous organs

(c) Homologous organs

(d) All of the above

Answer: (d) All of the above


Q38. The book "Origin of Species" was written by:

(a) Lamarck

(b) Darwin

(c) Mendel

(d) Hugo de Vries

Answer: (b) Darwin


Q39. Bt cotton is resistant to:

(a) Insects

(b) Bacteria

(c) Viruses

(d) Fungi

Answer: (a) Insects


Q40. The first transgenic cow produced:

(a) Human insulin

(b) Human growth hormone

(c) Alpha-lactalbumin (protein in milk)

(d) Hemoglobin

Answer: (c) Alpha-lactalbumin


Q41. RNA interference (RNAi) is used for:

(a) Gene silencing

(b) DNA replication

(c) Protein synthesis

(d) Mutation induction

Answer: (a) Gene silencing


Q42. Which disease is caused by a vitamin C deficiency?

(a) Scurvy

(b) Rickets

(c) Beriberi

(d) Pellagra

Answer: (a) Scurvy


Q43. Malaria is transmitted by:

(a) Anopheles mosquito

(b) Aedes mosquito

(c) Culex mosquito

(d) Housefly

Answer: (a) Anopheles mosquito


Q44. HIV attacks which cells in humans?

(a) RBCs

(b) WBCs (T-helper cells)

(c) Platelets

(d) Neurons

Answer: (b) WBCs (T-helper cells)


17. Ecology & Environment

Q45. The term "biodiversity hotspot" refers to:

(a) Areas with high species richness

(b) Areas with extreme temperatures

(c) Polar regions

(d) Deserts

Answer: (a) Areas with high species richness


Q46. The Montreal Protocol is related to:

(a) Climate change

(b) Ozone depletion

(c) Deforestation

(d) Wildlife conservation

Answer: (b) Ozone depletion


Q47. The 10% law in energy flow is associated with:

(a) Lindeman

(b) Darwin

(c) Mendel

(d) Odum

Answer: (a) Lindeman


18. Structural Organization in Animals

Q48. Which tissue connects bones to muscles?

(a) Ligament

(b) Tendon

(c) Cartilage

(d) Adipose tissue

Answer: (b) Tendon


Q49. The largest cell in the human body is:

(a) Neuron

(b) Ovum

(c) RBC

(d) WBC

Answer: (b) Ovum


Q50. The pH of gastric juice is approximately:

(a) 1.5–2.0

(b) 7.0

(c) 8.0

(d) 10.0

Answer: (a) 1.5–2.0


Q51. The hormone responsible for cell elongation is:

(a) Auxin

(b) Gibberellin

(c) Cytokinin

(d) Abscisic acid

Answer: (a) Auxin


Q52. Phototropism is a response to:

(a) Light

(b) Gravity

(c) Water

(d) Touch

Answer: (a) Light


Q53. Vernalization is related to:

(a) Flowering

(b) Seed germination

(c) Fruit ripening

(d) Leaf fall

Answer: (a) Flowering


20. Microbes & Human Welfare

Q54. Yeast is used in the production of:

(a) Bread

(b) Cheese

(c) Yogurt

(d) Vinegar

Answer: (a) Bread


Q55. The antibiotic penicillin is obtained from:

(a) Bacteria

(b) Fungi

(c) Algae

(d) Virus

Answer: (b) Fungi


Q56. Which bacterium is used in biogas production?

(a) Escherichia coli

(b) Methanobacterium

(c) Lactobacillus

(d) Rhizobium

Answer: (b) Methanobacterium


Q57. The normal blood pressure in humans is:

(a) 120/80 mmHg

(b) 140/90 mmHg

(c) 100/60 mmHg

(d) 160/100 mmHg

Answer: (a) 120/80 mmHg


Q58. The pacemaker of the heart is the:

(a) SA node

(b) AV node

(c) Purkinje fibers

(d) Bundle of His

Answer: (a) SA node


Q59. Which blood group is a universal donor?

(a) A

(b) B

(c) AB

(d) O

Answer: (d) O


Q60. The site of gas exchange in lungs is:

(a) Bronchi

(b) Alveoli

(c) Trachea

(d) Pharynx

Answer: (b) Alveoli


Q61. Hemoglobin has the highest affinity for:

(a) CO₂

(b) O₂

(c) CO

(d) NO

Answer: (c) CO


Q62. The diaphragm helps in:

(a) Digestion

(b) Respiration

(c) Circulation

(d) Excretion

Answer: (b) Respiration


23. Neural Control & Coordination

Q63. The neurotransmitter at neuromuscular junctions is:

(a) Dopamine

(b) Acetylcholine

(c) Serotonin

(d) GABA

Answer: (b) Acetylcholine


Q64. The part of the brain responsible for memory is:

(a) Cerebrum

(b) Cerebellum

(c) Medulla

(d) Hypothalamus

Answer: (a) Cerebrum


Q65. The reflex action is controlled by:

(a) Brain

(b) Spinal cord

(c) Both (a) & (b)

(d) Peripheral nerves

Answer: (b) Spinal cord


Q66. The functional unit of the kidney is:

(a) Neuron

(b) Nephron

(c) Alveoli

(d) Villi

Answer: (b) Nephron


Q67. ADH acts on:

(a) Heart

(b) Kidney

(c) Liver

(d) Lungs

Answer: (b) Kidney


Q68. The main nitrogenous waste in humans is:

(a) Ammonia

(b) Urea

(c) Uric acid

(d) Creatinine

Answer: (b) Urea


Q69. Bile is produced by:

(a) Liver

(b) Pancreas

(c) Stomach

(d) Small intestine

Answer: (a) Liver


Q70. The enzyme pepsin digests:

(a) Carbohydrates

(b) Proteins

(c) Fats

(d) Nucleic acids

Answer: (b) Proteins


Q71. Villi are present in:

(a) Stomach

(b) Small intestine

(c) Large intestine

(d) Esophagus

Answer: (b) Small intestine


Q72. Binary fission is seen in:

(a) Amoeba

(b) Humans

(c) Earthworms

(d) Plants

Answer: (a) Amoeba


Q73. Parthenogenesis is a type of:

(a) Sexual reproduction

(b) Asexual reproduction

(c) Vegetative propagation

(d) Fertilization

Answer: (b) Asexual reproduction


Q74. The male reproductive part of a flower is:

(a) Pistil

(b) Stamen

(c) Petal

(d) Sepal

Answer: (b) Stamen



Q75. The structure of DNA was discovered by:

(a) Mendel

(b) Watson & Crick

(c) Darwin

(d) Lamarck

Answer: (b) Watson & Crick


Q76. The process of copying DNA is called:

(a) Transcription

(b) Translation

(c) Replication

(d) Mutation

Answer: (c) Replication


Q77. mRNA carries the genetic code from:

(a) DNA to ribosomes

(b) Ribosomes to DNA

(c) Nucleus to cytoplasm

(d) Cytoplasm to nucleus

Answer: (a) DNA to ribosomes



Q78. Restriction enzymes are used in:

(a) DNA cutting

(b) DNA synthesis

(c) Protein synthesis

(d) RNA splicing

Answer: (a) DNA cutting


Q79. Gel electrophoresis separates DNA based on:

(a) Charge

(b) Size

(c) Color

(d) Shape

Answer: (b) Size


Q80. The Ti plasmid is used in genetic engineering of:

(a) Animals

(b) Plants

(c) Bacteria

(d) Fungi

Answer: (b) Plants



Q81. A group of individuals of the same species is called:

(a) Community

(b) Population

(c) Ecosystem

(d) Biome

Answer: (b) Population


Q82. An example of a mutualistic relationship is:

(a) Lichen

(b) Predation

(c) Parasitism

(d) Competition

Answer: (a) Lichen


Q83. The study of interactions between organisms and their environment is:

(a) Genetics

(b) Ecology

(c) Evolution

(d) Physiology

Answer: (b) Ecology


30. Strategies for Enhancement in Food Production

Q84. Inbreeding is used to:

(a) Increase heterozygosity

(b) Increase homozygosity

(c) Introduce mutations

(d) Reduce yield

Answer: (b) Increase homozygosity


Q85. MOET stands for:

(a) Multiple Ovulation Embryo Transfer

(b) Molecular Orbital Energy Transfer

(c) Mitochondrial Oxidative Enzyme Test

(d) None of the above

Answer: (a) Multiple Ovulation Embryo Transfer


Q86. Apiculture is related to:

(a) Fish farming

(b) Bee keeping

(c) Poultry

(d) Dairy

Answer: (b) Bee keeping


Q87. Eutrophication is caused by excess:

(a) CO₂

(b) Nitrogen & Phosphorus

(c) Oxygen

(d) Heavy metals

Answer: (b) Nitrogen & Phosphorus


Q88. The "Chipko Movement" was related to:

(a) Water conservation

(b) Forest conservation

(c) Air pollution

(d) Wildlife protection

Answer: (b) Forest conservation


Q89. The main cause of ozone depletion is:

(a) CO₂

(b) CFCs

(c) SO₂

(d) NO₂

Answer: (b) CFCs


Q90. The hormone that regulates sleep is:

(a) Melatonin

(b) Insulin

(c) Thyroxine

(d) Adrenaline

Answer: (a) Melatonin


Q91. The largest gland in the human body is:

(a) Pancreas

(b) Liver

(c) Thyroid

(d) Pituitary

Answer: (b) Liver


Q92. The "Father of Indian Green Revolution" is:

(a) M.S. Swaminathan

(b) C.V. Raman

(c) Hargobind Khorana

(d) A.P.J. Abdul Kalam

Answer: (a) M.S. Swaminathan


Q93. A dihybrid cross ratio is:

(a) 3:1

(b) 9:3:3:1

(c) 1:2:1

(d) 1:1:1:1

Answer: (b) 9:3:3:1


Q94. Klinefelter’s syndrome has the genotype:

(a) 45, X

(b) 47, XXY

(c) 47, XYY

(d) 46, XY

Answer: (b) 47, XXY


Q95. The process of RNA synthesis from DNA is:

(a) Replication

(b) Transcription

(c) Translation

(d) Mutation

Answer: (b) Transcription


Q96. The hormone secreted by the adrenal medulla is:

(a) Cortisol

(b) Adrenaline

(c) Insulin

(d) Thyroxine

Answer: (b) Adrenaline


Q97. The part of the brain controlling hunger is:

(a) Cerebellum

(b) Hypothalamus

(c) Medulla

(d) Thalamus

Answer: (b) Hypothalamus


Q98. The vitamin synthesized by gut bacteria is:

(a) Vitamin A

(b) Vitamin B12

(c) Vitamin C

(d) Vitamin D

Answer: (b) Vitamin B12


Q99. The C4 pathway is seen in:

(a) Wheat

(b) Rice

(c) Maize

(d) Potato

Answer: (c) Maize


Q100. The pigment involved in photoperiodism is:

(a) Chlorophyll

(b) Phytochrome

(c) Xanthophyll

(d) Carotene

Answer: (b) Phytochrome




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